Part:BBa_K562008
Salty_PduABTUN BMC Components
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA, PduB, PduT, and PduU proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562007) AND protein PduN. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB, PduU and PduN gene products carry C-terminal 6-His affinity/epitope tags. Production of the PduB, PduU and PduN proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT, PduU and PduN have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTUN in the Sargent Laboratory, Dundee, UK.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1898
Illegal BamHI site found at 2490 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1441
Illegal AgeI site found at 1426
Illegal AgeI site found at 2040 - 1000COMPATIBLE WITH RFC[1000]
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